Microfluidics can precisely control the physiochemical properties of nanoparticles by mixing to the nanoliter. For this reason, nanoparticles for the transport of peptide drugs are to be developed in the study presented here with the help of chitosan from Heppe Medical Chitosan GmbH.
The preparation of chitosan-based nanoparticles by ionotrophic gelation often leads to high batch-to-batch variability as well as in low reproducibility. A possible solution to this could be controlled mixing on a microfluidic scale. In the study presented here, chitosan tripolyphosphate nanoparticles with siRNA, mRNA and β-galactosidase as cargo will be prepared using microfluidics.
More than 5 million people worldwide are affected by inflammatory bowel disease. To enable good treatment, targeted and efficient drug delivery is necessary. In the presented study, a chitosan-based vancomycin loaded microgel was investigated for this purpose.
In microfluidic systems, analyses can be performed on a miniaturized scale without the use of complex laboratory equipment. In this context, DNA purification and concentration pose a particular challenge. To improve this, a disposable microfluidic device is functionalized with different chitosans for DNA binding in the presented study. This will then be used to detect eDNA of invasive species from river water samples.
