Fluorescence immunoassay for detection of pathogens
Listeriosis is a foodborne infectious disease caused by the pathogen Listeria monocytogenes and is particularly serious for pregnant women and immunocompromised persons. The pathogen is spread mainly through spoiled and contaminated food or animal feed. Methods for the detection of Listeria monocytogenes (LM) on surfaces in the food industry have been developed to control the spread of the pathogen, but these are time-consuming and require special equipment. In the presented study, a fluorescence sandwich immunoassay was developed using a chitosan cellulose nanocrystal (CNC) membrane to detect Listeria monocytogenes on surfaces.